Purpose
· The purpose of
BSL -2/Polio requirement is to reduce the risk of reintroducing wild polio
viruses from the lab. in to the community at a time, when polio virus
circulation is decreasing or no longer occurring in many areas of the world and
prevent infection of the personnel and contamination of the materials used in
the polio virus isolation and identification.
Definition
- BSL- 2/Polio refers to standard BSL – 2 conditions with additional specific requirements for wild polio viruses.
Material
- A separate BSL – 2 cabinets for each lab.
- Waste generated from the procedure done in each Bio-safety cabinet is collected in a closed container with 1% Sod. Hypochlorite.
- After the day’s work, every hypochlorite container is autoclaved at 121* C for 30 minutes.
Procedure
· BSL – 2/ Polio
includes the following precautions specifically for lab. holding wild
poliovirus materials.
· Operational
practices
o
Access
to labs is restricted.
o
Eating,
drinking and applying cosmetics is not permitted in the laboratory.
o
All
persons entering in the laboratory including support staff ie: cleaners,
maintenance personnel etc are maintained with one dose of OPV
o
Accurate
records of wild polio viruses stock are maintained (For records Refer to
Register PR-19)
o
(After ITD results are submitted, then within
three months all isolates and sample extracts containing wild virus are
discarded by autoclaving.)
· Storage
o
Freezers
containing polio virus isolates are locked and there is limited access to the
key mechanism.
o
A
detailed current inventory of all contents of freezers and documentation of all
withdrawal and addition is maintained in log book of the particular freezers.
·
Transfer of materials
o
Great
care is taken to avoid spillages and breakages when wild virus infectious and
potential infectious material are moved from the freezers.
o
All
material with wild polioviruses or infectious materials are transferred only in
leak proof unbreakable secondary containers that can be disinfected if spillage
occurs.
o
SOP
to avoid cross contamination.
SPECIAL PRACTICE
· In general
persons whom infection may be unusually hazardous for example immuno –
compromised or immunosuppressed persons are not allowed in the lab
· Laboratory
personnel receive appropriate immunizations.
· Biosafety manual
is prepared.
· Laboratory
equipment and work surfaces should be decontaminated with appropriate
disinfection on a routine basis, after work with infectious materials are
finished.
·
Spills and
accidents are immediately reported to the in charge of the lab
SAFETY
EQUIPMENTS
- Safety equipment includes biological safety cabinets (BSC) enclosed containers and other engineering controls designed to remove or minimize exposure to hazardous biological materials. The BSC is the principal of device used to provide containment of infectious splashes or aerosols generated by many microbiological procedures.
- Safety equipment also may include items of personal protection such gloves, coats, shoes, covers, face shields, safety gloves etc.
- Properly maintained biosafety cabinets (Class II Refer SOP No.7)
- Face protection is used.
- Protective laboratory coats, gowns, are worn in the laboratory.
· Gloves are must be worn when handling infectious
materials.
LABORATORY FACILITY
- The design of the facility is important in providing a barrier to protect persons working inside & outside of the laboratory within the laboratory & to protect persons in the community from infectious agents, which may be accidentally released from the laboratory.
- Each lab. Contain a sink for hand washing
- Lab. is designed so that it can be easily cleaned.
- Bench tops are impervious to water & resistant to acids, alkalis & organic solvents and moderate heat.
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